Subcutaneous sc or sq injection in rats and mice sop. Pdf intraperitoneal injection of tetracyclines protects. Injection of syngeneic murine melanoma cells to determine. All injections must be described in your approved animal study proposal. B the dead mice teemed with living pathogenic r cells. J p steward, e p ornellas, k d beernink, and w h northway. The continuous estradiol group contin e2 was injected daily with. Disinfect the site of injection and insert the needle directly into the tumor, and penetrate as deeply as possible without passing through the tumor.
This is consistent with the trend of greater effect on the nervous system of. We report a method for the establishment of pulmonary metastases in mice, useful for the study of this phenomenon. Intravascular injection of neonatal mice also effectively. How do drugs get into the circulation by intraperitoneal injection. Vehicletreated mice received daily injections of 2hydroxypropyl. However, pristane also induces plasmacytomas and an erosive arthritis resembling rheumatoid arthritis in balbc mice, probably as a consequence of enhanced interleukin 6 production. Intraperitoneal ip injection in rats and mice sop page 4 of 6 mouse rat 6. The late late show with james corden recommended for you. Injected substances and the needlessyringes used to inject substances must be sterile.
Intraperitoneal injection in mice research at penn state. C the killer strain r was encased in a protective capsule. For neonatal mice, like adult mice, a righthanded operator will find it easiest to place the pup in left lateral recumbency, with its head facing to the right, and administer the injection into the right retroorbital sinus. Injection sites and volumes use the smallest size needle gauge possible when performing injections. When compared with traditional antibodybased brdu methods, the clickit plus edu cell proliferation assays not only offer better performance and an easier workflow but are now compatible with an even broader range of commonly used fluorescent probes, including gfp, rfp, and other fluorescent proteins as well as phycobiliproteins. Intraperitoneal ip injection in rats and mice sop ubc animal. Fviii null or vwf null mice were infused with plasma. In a series of 42 mice injected in this fashion, 36 mice were judged to have received an excellent injection, 3 received a good injection, 2 received an ok injections and only 1 animal a poor injection. The laboratory mouse handbook of experimental animals. Nov 27, 20 generation of mutant mice by pronuclear injection of circular plasmid expressing cas9 and single guided rna skip to main content thank you for visiting. Generation of mutant mice by pronuclear injection of circular. Click the image below to see a chart it is important that you discuss the appropriate route, volume, site and needle selection with your veterinarian.
Intraperitoneal injection an overview sciencedirect topics. Errors ranging from 21 to 28% were obtained, independent ofthree investigators at two different times, all using. Get like 1020 cd1 mice, be sure to warm up mice with a 250 w infrared lamp at about 1 ft distance until their tail vein are real dilated. Evaluation of a novel technique for intraperitoneal injections in mice article pdf available in lab animal 4411. Induction of lupusassociated autoantibodies in balbc. In mice, strain, body weight, age, and sex add to the wellknown anesthetic variability that exists among individuals of other species. Potential complications for all routes of injection include infection, local irritation, pain and damage to surrounding tissue. Tumor development in nunu mice on injection of hek293 openi. Considering the risks and safety of intraperitoneal injections. Get a printable copy pdf file of the complete article 285k, or click on a page image below to browse page by page. Increased erythropoiesis in mice injected with submicrogram. This is a handout that accompanies a handson mouse biomethodology. At the end of experiment, mice are euthanized and selected tissues are analyzed by ex vivo. If there is negative pressure, proceed with the injection.
Imaging procedure mice are injected by an intraperitoneal route with a luciferin solution 15 mgml or 30 mgkg, in pbs, dose of 150 mgkg that is allowed to distribute in awake animals for about 515 minutes. Pharmacological inhibition of type i interferon signaling protects mice against lethal sepsis lien dejager, 1,2so. Urethane administered to mice by intraperitoneal injection caused tumors of the thymus thymoma in both sexes and the blood vessels in females, in addition to liver and lung tumors, as observed in earlier studies dahl et. Those trained persons listed on an approved animal care committee protocol performing the procedure. We explored whether infusion of vwf and fviii by either intraperitoneal i. Intraperitoneal injections science topic forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall. The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible. The pharmacokinetic behaviour of docetaxel was similar in control mice and cancer. As an example, mice less than 8 weeks of age metabolize anesthetics less efficiently than adults due to their immature liver enzymatic system and reduced homeostatic response. Pharmacological inhibition of type i interferon signaling. Inhibition of human tumor xenograft growth in nude mice by a. The mice are placed into a clear plexiglass anesthesia box 2. Some sites, such as footpad injection of freunds complete adjuvant, intrasplenic injection and.
Making lives easier for animals in research labs, animal welfare institute, washington, dc, 2007. Mice are placed onto black paper in the ivis imaging box and imaged dorsally. Mice, injected with a single dose of tetracycline base tetb. Recommended needle sizes and injection sites for various species are indicated. Only the tip of the needle should penetrate the abdominal wall, to prevent injection into the intestine waynforth, 1980. Incorporation of brdu can be detected in thymus and bone marrow in as little as 1 hr post injection.
Instruct participants in substance administration to include intramuscular im. Procedure summary and goal describes procedure for the administration of fluids or compounds into the abdominal cavity. Intravascular delivery of viral vectors into the superficial temporal facial vein has been reliably used in various studies in neonatal mice to treat the pediatric neuromuscular disorder spinal muscular atrophy sma 2,4,14 and ultimately increased the lifespan of treated mice. Find someone appropriately trained in this procedure to help before proceeding to the animal house. Another example of poor efficacy after intraperitoneal administration due to a first pass effect is seen with various biogenic amines. Full text full text is available as a scanned copy of the original print version.
Baumans v, coke c, green j, moreau e, petersonkane e, reinhardt a, reinhardt v, van loo p eds. The humane society of the united states euthanasia reference manual vii acknowledgments the previous edition of this manual was authored by rebecca h. Based on the results showing that mab1e41c2 specifically reacted with hepg2, human hepatocellular carcinoma hcc cell line hepg2, we inhibition of human tumor xenograft growth in nude mice by a novel monoclonal antihspg isolated from human liver. Intraperitoneal injection in the mouse procedures with care. Experimental manipulation of pituitary hemorrhage induced. This policy addresses issues related to administration of compounds within a protocol with the goal of facilitating the procedure for the person administering the compound as well as minimizing discomfort and. Efficient in vivo gene transfer by intraperitoneal. Intraperitoneal injection is a common method of administering drugs to rodents. For instance, one of the earliest published murine models of stec infection relied on the subcutaneous injection of large quantities 10 8 cfu of e. Tail vein injection of b57bl6j mice with b16bl6 is among the most used models for melanoma metastases. C every other day between 16 and 18 weeks of age aggravated lupus nephritis in dsrnatreated mrl lprlpr mice 30. Intraperitoneal injection and recovery of vwf and fviii. An intraperitoneal injection should avoid injecting the organs. L, the suspension of calcium microflowers containing naked pdna mf, 6.
Mouse injections biomethodology for laboratory mice. Intraperitoneal injection of synthetic dsrna poly i. As expected, the intraperitoneal drug concentration was much. Get a printable copy pdf file of the complete article 1. Mouse intravenous injection handout mcgill university.
A 10 mgml solution of brdu in sterile 1x dpbs is provided for in vivo use. Four weeks post injection, mice were sacrificed and perfused with 4% paraformaldehyde. An excellent injection is noted to have direct visualization of all injected material without evidence of extravasation. Im injections are not recommended and should be avoided whenever possible due to the lack of muscle mass in the mouse. To provide additional warmth for the mice, we turn on the stage light in the microscope and cover it with a gauze pad. The laboratory mouse handbook of experimental animals usp. Mice were sacrificed within 15 to 30 minpost injection. We purify dna fragment for microinjection from plasmid dna provided by the customer. For adult rodents and larger animals, intravenous injections are technically feasible and routine. The injection of substances directly into the body requires strict asepsis to avoid complications. Guidelines on anesthesia and analgesia in mice research.
This policy addresses issues related to administration of compounds within a protocol with the goal of facilitating the procedure for the person administering the compound as well as minimizing discomfort and supporting wellbeing of the animal. Then, purified dna fragment is microinjected into 200 fertilized eggs of c57bl6 mouse strain for producing founder mice f0. Intraperitoneal injection of ethanol for the euthanasia of. Miner, jacqueline koehler, and laura greenaway department of infectious diseases, human research and development laboratories, dow chemical company, indianapolis, indiana 46077. Errors in the technique of intraperitoneal injection of mice. Here we present an overview of the methods used to deliver tamoxifen to mice. Despite the prevalence and relative harm of metastasis, therapeutics for treatment or prevention are lacking.
As with all injection sites, using a new needle for each animal, and injecting fluid that is at body temperature will reduce any discomfort caused by the procedure. When fred griffith injected mice with a mixture of dead. Inhibition of monoamineoxidase mao potentiates strongly the pressor effect when the drug is given by the intraperitoneal route. Full text is available as a scanned copy of the original print version. The intramuscular administration is not recommended. An increase in proteinuria, glomerular necrosis, chemokine production, and inflammatory cell infiltration was found in the kidney of dsrnatreated mrl lprlpr mice. Intraperitoneal ip injections should be made into the animals right abdominal quadrant so as to avoid penetrating the cecum on the left side. The injection site should be in the lower left quadrant of the abdomen because vital organs are absent from this area. Experimental manipulation of pituitary hemorrhage induced by intraperitoneal injection of a hypertonic solution in mice by chie iga. Needle size example 22 gauge is larger than 24 gauge recommended max dose subcutaneous scruff, 40 mlkg 0r 0. Results of intraperitoneal and oral administration of thymoquinone in mice are given in tables1a and 1b, respectively.
Intraperitoneal injection or ip injection is the injection of a substance into the peritoneum body cavity. Nov 11, 2014 intravenous injection is a clinically applicable manner to deliver therapeutics. Experimental manipulation of pituitary hemorrhage induced by intraperitoneal injection of a hypertonic solution in mice. Material that is irritant or with a high or low ph can cause pain both during and following injection. Retroorbital injection in the mouse research at penn state. Get a printable copy pdf file of the complete article 480k, or click on a page image below to browse page by page. Effects of continuous and intermittent estrogen treatments on. Method to perform iv injections on mice using the facial vein. Intraperitoneal injection of lipopolysaccharide lps. All animal users performing ip injections in rodents must. Intraperitoneal injection ip injection is a common used method in animal study. Briefly, mice were restrained by firmly grasping the skin over the dorsal neck by using the thumb and forefinger of the handlers nondominant hand, with the tail held between the palm and ring finger of the same hand. Characterization of intraperitoneal, orthotopic, and.
Links to pubmed are also available for selected references. It is more often applied to animals than to humans. The injection of 3g recombinant murine epo could not be detected beyond 6 hours postinjection, suggesting a rapid halflife, in agreement with studies that found a halflife for exogenously delivered epo in mice of about 2 hours. Optimization of intraperitoneal injection anesthesia in mice. Pdf evaluation of a novel technique for intraperitoneal. Lab techniques in mice free download as powerpoint presentation. Sep 23, 2015 these data suggest that the injection procedure itself is associated with little or no aversion particularly in wellhandled animals and that the major stressor is the restraint used to. Multiplex your cell proliferation assays with gfp, rfp, and r. H7 was explored as a system to assess potential differences in virulence of. In contrast, neither vwf nor fviii was detected in the plasma following sq injection. Tilt the mouse with its head slightly toward the ground so that its head is lower than its hind end. Im injections are administered in the thigh muscles of the hind limb.
The most accessible vessels for intravenous administration in rodents are veins that run the length of both lateral aspects of the tail. Good practice administration routes and volumes for mice. Intraperitoneal injection of tetracyclines protects mice from lethal endotoxemia downregulating inducible nitric oxide synthase in various organs and cytokine and nitrate secretion in blood. Subcutaneous sc or sq injection in rats and mice sop page 2 of 2 procedure 1. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Administration of drugs and experimental compounds in mice. Intraperitoneal ip injection in rats and mice sop page 5 of 6 for mice.
Jci acetaminopheninduced hepatotoxicity in mice is. Prepare the syringe by placing the needle on the syringe. Sep 22, 2012 when fred griffith injected mice with a mixture of dead, pathogenic, encapsulated s cells and living unencapsulated r cells of streptococcus pneumoniae, he discovered that a the previously harmless strain s had inherited the ability to kill mice. The ld 50 of thymoquinone in rats was determined to be 57.
Good practice administration routes and volumes for mice, rats, and rabbits are indicated in table 1. Retroorbital injections in mice europe pmc article. Intraperitoneal injection of lipopolysaccharide induces. All injections must be performed using sterile needles and syringes. The sciatic nerve runs along the caudal back length of the femur and must be avoided.
Considering the risks and safety of intraperitoneal injections article pdf available in lab animal 454. Results using 165 mice, 24%received the ip injection in a place other than the peritoneal cavity. Signal at the injection site will be detectable at day 0. Administration of compounds plays a large part in experimental design using animals. We have tested whether tetracyclines tets are able to protect mice from lipopolysaccharide lpsinduced shock, a cytokinemediated inflammatory reaction. This allows the abdominal viscera to shift cranially and minimize accidental puncture of abdominal organs at site of injection. Tumor development in nunu mice on injection of hek293 cells stably transfected with pcdna3. Pharmacokinetic advantage of intraperitoneal injection of.
In the control groups, 10 mice were not subjected to any kind of treatment sham group and 20 mice received one intraarticular injection of 20. Significant serum interferon titers were attained in swiss mice during a 24hr period after intraperitoneal injection of 10 to 300 mug of polyi. When the subcutaneous tumor areas reach approximately 3. How do drugs get into the circulation by intraperitoneal.
Injection of vascular endothelial growth factor into knee. Intravenous injection subcutaneous injection mouse. Over 90% of animals used in research are rats and mice, and this policy deals only with rats and mice. Intraperitoneal injections are used to administer solutions into the intraperitoneal cavity of the mouse the cavity that encloses the stomach, kidneys, liver and other abdominal organs. Hitting the sciatic nerve can cause pain, lameness, and possible paralysis. Intravenous injection of mice with spontaneouslyderived motility mutants of e. However, some mouse models can have early onset of disease with a rapid progression that makes administration of potential therapies difficult. Intramuscular injection in the rat procedures with care.
Lateral tail vein injection in mice and rats ucsf iacuc. Pull back on the plunger to ensure negative pressure prior to injecting. Intraperitoneal injection 42 was performed by a single researcher kaw. Some sites, such as footpad injection of freunds complete adjuvant, intrasplenic injection and intra lymph node injection. Both vwf and fviii were absorbed into the blood circulation after ip injection with a peak between 2 to 4 hours with levels similar to those observed in mice infused intravenously. Briefly, mice were restrained by firmly grasping the skin over the dorsal neck by using the thumb and forefinger of the handlers nondominant hand, with. From a subset of mice, plasma was collected prior to the cancer cell injection and once weekly throughout the experiment via the saphenous vein. Errors related to different techniques of intraperitoneal. Intraperitoneal injection of pristane 2,6,10,14 tetramethylpentadecane is a standard technique for obtaining monoclonal antibodyenriched ascitic fluid. Pdf intraperitoneal injection is a common technique that safely delivers a substance into the peritoneal cavity but can induce high stress in animals find, read and cite all the research. Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence.
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